PREMIER TECHNOLOGY DEVELOPMENT AND SERVICE CENTER FOR COCOA BIOTECHNOLOGY

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MOLECULAR BIOLOGY: WORKING WITH DNA

ISOLATION

1. Acid Phenol Extraction Purification of Supercoiled DNA
2. Agarose Gel Electrophoresis of DNA
3. Alkali Plasmid DNA Maxiprep Using Cesium Chloride Cushion
4. Alkali Plasmid DNA Miniprep Using Ammonium Acetate Precipitation
5. Alkali Plasmid DNA Miniprep Using Phenol:Chloroform
6. Alkaline Lysis Method of Plasmid DNA Preparation
7. Alkaline Lysis Plasmid DNA Miniprep
8. Ammonium Chloride Lysis of Red Blood Cells for Collection of White Blood Cells for DNA Extraction
9. Apoptosis Assay using DNA Laddering
10. Boiling Miniprep for Plasmid DNA
11. Boiling Preparation of Plasmid DNA for Sequencing
12. Cesium Chloride Gradient Purification of Plasmid DNA
13. Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
14. Determination of DNA Base Composition by Acid Hydrolysis
15. Diphenylamine Assay for DNA
16. Distillation of Phenol
17. DNA Quantitation Using PicoGreen Dye
18. Elution of DNA Fragments from Agarose Gels Using NA45 Paper
19. Extraction of DNA from Low Gelling Temperature Agarose
20. Extraction of Nuclear DNA from Cultured Cells
21. Fast Mouse Genomic DNA Purification
22. Fluorometric Determination of DNA Content
23. Hoechst Fluorometric Quantification of dsDNA.
24. Isolating DNA Fragments from an Agarose Gel
25. Large Scale Cesium Chloride Plasmid DNA Preparation
26. Large-Scale Plasmid DNA Isolation from Bacteria by the Alkaline Lysis
27. Ligation in Low-Melting Temperature Agarose Gel
28. Mega DNA Plasmid Preparation
29. Modified Alkaline Lysis Plasmid Miniprep
30. Mouse Tail Tip Digest Protocol: PCR from Tail Tip DNA
31. Mouse Tail Tip Digest Protocol: Preparation of DNA
32. Neutralization and Equilibration of Phenol
33. Phage DNA Purification with DE52 Cellulose
34. Plasmid DNA Prep Using Cesium Chloride Gradient and Dowex Column
35. Preparation of Genomic DNA from Transfected ES Clones
36. Preparation of Glassmilk for DNA Purification
37. Preparation of Herring Sperm DNA (HSS-DNA) for Yeast Transformation
38. Preparation of High Molecular Weight DNA from Zebrafish
39. Preparation of P1 DNA
40. Preparation of Single Stranded Plasmid DNA
41. Preparation of Uracil Containing Single Stranded DNA for Site-Directed Mutagenesis
42. Preparation of Yeast Cells for DNA Quantitation by Fluorescence-Activated Cell Sorting (FACS)
43. Preparation of Yeast Cells for FACS DNA Quantitation
44. Preparation of Yeast DNA Embedded in Agarose Plugs for Pulsed Field Gel Electrophoresis (PFGE)
45. Processing of Microdissected Tissue for Molecular Analysis: DNA-based Studies, RNA-based Studies, and Protein-based Studies
46. Purification of DNA from Agarose Gels Using Glassmilk
47. Purification of Large E. Coli Restriction Fragments Using Field Inversion Gel Electrophoresis (FIGE)
48. Purification of Oligonucleotides by Thin Layer Chromatography (TLC)
49. Purification of Yeast Genomic DNA
50. Purifying DNA from Transformed Yeast Using Glass Beads and Phenol
51. Purity Measurement and Quantification of DNA
52. Quantification of DNA Samples
53. Rapid Elution of DNA from Agarose Gels
54. Rapid Rescue of Yeast Plasmids Using Glass Beads and Phenol
55. Rapid Small-Scale Isolation of Drosophila DNA
56. Sephadex G-50 Spin Column DNA Purification
57. STET Method of DNA Isolation (Boiling Miniprep)
58. Yeast Plasmid Rescue for Multiple Cultures



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